Pengaruh Pemberian Minyak Buah Merah (Pandanus conoideus) Sebelum dan Selama Infeksi Plasmodium berghei ANKA terhadap Sel Leukosit Mononuklear Mencit Swiss

Abstract

Background: Lymphocyte is a mononuclear cell which has important roles in malaria parasite elimination but recent studies have shown that the apoptosis level of mononuclear cells in peripheral blood increased during acute malaria. Consumption of 30-60 milligrams beta-carotene each day in two months will increase lymphocyte’s activities. Red fruit oil contains high levels of beta-carotene. This study compared the effect of red fruit oil with the percentage of mononuclear cells between treated and control group before and during infection. Methods: It was a Pre and Post Test Control Group Design experimental study. The samples were 14 mice with specific criteria and divided into two groups: control group and treated group (treated by 0,05 cc/day P. conoideus oil). Both groups were infected with 0,1 cc mice blood which contained 1 x 104 P. berghei ANKA. The percentage was counted each day by thin blood smear examination using 400x microscope. Results: On day 10 and 13, treated group’s percentage was significantly higher than control (day 10 p=0,003; day 13 p=0,002). The treated group’s percentage was lower than control on day 15 and 18. It was significant on day 15 (p=0,006) but not on day 18 (p=0,345). Conclusion: P. conoideus oil significantly increases the mononuclear cells’ percentage in treated group before infection but not during infection. Keyword: Pandanus conoideus, mononuclear cell, Plasmodium berghei ANKA