Pengaruh Pemberian Minyak Pandanus conoideus Terhadap Derajat Parasitemia Mencit Swiss Yang Diberikan Sebelum Diinfeksi Plasmodium berghei ANKA

Abstract

Background: Pandanus conoideus Lam. (buah merah) is a well-known advantageous herbal from Papua, contains active substances that can increase Natural Killer proliferation in the human body. According to the observation of the P. conoideus’ inventor on the native of Papua who consumed P. conoideus in the certain period of time, they became stronger and able to survive from disease. As we know, Papua is one of severe endemic area of malaria. For this reason, the researcher tries to evaluate the effect of P. conoideus Lam pretreatment on parasitemia degree of Plasmodium berghei-infected Swiss mice. Method: This was an experimental study using post test-only control group design with 14 female mice, 25-30 grams, 8-12 weeks, randomized into 2 groups. Both groups were given standard dietary for a week. At days 8th, group I (K) was infected by Plasmodium berghei ANKA. Group II (P) was given Pandanus conoideus oil for five days ( days 8th until days 12th), At days 13th, it was infected by P. berghei ANKA, then continued giving P. conoideus oil from days 14th until days 18th. Some peripheral-blood samples of group I were taken from mice’s tail for making preparat at days 9th until days 13th, and for group II, at days 14th until days 18th. Parasitemia degree was examined by counting the number of parasitized erythrocytes per 1000 cells and analyzed using Saphiro-Wilk and independent t-test. Results: The mean of parasitemia degree of group I is 31,74 ± 11,03 and group II is 13,36± 5,17. Parasitemia degree of group II is lower than group I. There was a significant difference of parasitemia degree between group I and group II, analyzed by independent t-test at says 1st, 3rd, and all days of experiment, but not significant at days 5th of experiment. Conclusions: The administration of Pandanus conoideus Lam, before Plasmodium berghei ANKA infection, may reduce the parasitemia degree of Swiss mice. Key words: Pandanus conoideus Lam., parasitemia degree, Plasmodium berghei ANKA.