PENGARUH PEMBERIAN EKSTRAK MENIRAN (Phyllanthus sp. )TERHADAP GAMBARAN MIKROSKOPIK HEPAR TIKUS WISTAR YANG DIINDUKSI KARBON TETRAKLORIDA

Abstract

Background : Meniran ( Phyllanthus sp. ) has been used for a long time as a traditional medicine. Meniran contains flavanoid as antioxidant which was able to inhibit free radicals. Carbon tetrachloride is a chemical substance that toxic to the liver. The metabolism of CCl4 produce free radical CCl3- that can damage liver cell. The purpose of this research was to see the effect of meniran extract to the microscopic pattern of liver that has been induced by carbon tetrachloride. Methode : This research was experimental study which used post test only with control group design, with 27 female Wistar rats which devided into three groups. Group I was control group, group II induced by CCl4 1% 1 ml/day in sesame oil oral dose, group III induced by CCl4 1% 1ml/day in sesame oil oral dose and meniran extract 1% 2 ml/day oral dose for 21 days. The rats were terminated at 30th day and then microscopic pattern of Wistar rat’s liver was examinated. Data were analyzed by Kruskal-Wallis and Mann-Whitney test. Result : There was a significant different in normal hepatocyte count between group K-P1 (p<0,001), K-P2 (p<0,001), P1-P2 (p<0,001), steatosis between K-P1 (p<0,001), K-P2 (p<0,001), necrosis between K-P1 (p<0,001), K-P2 (p=0,046), P1-P2 (p<0,001). But there was no significant different in steatosis count between P1-P2 (p=0,199) Conclusion : Meniran extract had significant effect on decreasing necrotic hepatocyte induced by CCl4 1%. But there was no significant effect on decreasing steatosis Keyword : Microscopic pattern of liver, carbon tetrachloride, meniran extract