Pengaruh Ekstrak Syzygium polyanthum Terhadap Produksi Nitrit Oksida (NO) Makrofag Pada Mencit Balb/c Yang Diinokulasi Salmonella typhimurium

Abstract

Background: The extract from salam (Syzygium polyanthum) is one of herbal medicine used to treat various diseases and it has been used in many countries. Syzygium polyanthum contains many substances such as atsiri, tannin, flavonoid and metachavicol that can help bacterial killing and increasing immune response by enhancing phagocytosis. In phagocytosis, activated phagocyte (macrophage and neutrophyl) will secrete NO. The objective of this study is to find the effect of Syzygium polyanthum as immunomodulation. It is showed by the raise of nitric oxide production by macrophage of Balb/c strain mice inoculated by Salmonella typhimurium. Methode: This study was an experimental study, using The Posttest Only Control Group Design. The sample were 20 Balb/c mice and devided into 4 groups randomly. They were: K (no treatment), K+ (inoculated with Salmonella typhimurium), DS (inoculated with Salmonella typhimurium and treated with Syzygium polyanthum extract with dose 56,7 mg, 1 ml/day) and TH (inoculated with Salmonella typhimurium and treated with green tea extract). Inoculation at the first day as much as 106, 5 days later the mice killed. Macrophage isolated from peritoneal cavity and then the nitric oxide was measured. Results: There was significant difference (p< 0,05) on the number of macrophage nitric oxide production on the DS group and TH group compare to other groups (K and K+). Conclusion: The Syzygium polyanthum treatment with dose 56,7 mg, 1 ml/day peroral to Balb/c mice inoculated by Salmonella typhimurium raise the nitric oxide production of macrophage. Keywords: Syzygium polyanthum, nitric oxide, Salmonella typhimurium, macrophage.