PENGARUH PEMBERIAN RUMPUT MUTIARA (Hedyotis corymbosa)DENGAN DOSIS BERTINGKAT TERHADAP GAMBARAN HISTOLOGI GINJAL MENCIT BALB/C

Abstract

Background : Rumput Mutiara (Hedyotis corymbosa) is one of the herbal medicines used empirically by Indonesian people to treat diseases or to maintain their health. Its originated products have been sold in wide variety of doses. People use it for a long period of time. In the body, Hedyotis corymbosa will be absorbed, distributed, metabolized and excreted. Kidney is the primary organ of excretion, so it is often damaged by toxic substances. The purpose of this experiment is to evaluate the changes of histological appearance of Balb/c mice’s kidneys after the administration of gradual doses of Hedyotis corymbosa for 14 days. Method : This was experimental study using the post test only control group design. The samples were 20 Balb/c mice with specific criteria and randomized into 4 groups. The Control group (K) received water only, while group P1,P2,P3 were given the extract of Hedyotis corymbosa in gradual doses for 14 days, respectively 80 mg, 160 mg, and 320 mg. Results: There were proximal tubule damages i.e edema in almost every group extend from mild to severe. Using One Way ANOVA test, there was a significant difference between groups (p<0.05), continued with Post Hoc test, there was a significant difference (p< 0,05) between groups: K and P1-3; P1 and P3; P2 and P3, but no significant difference ( p>0.05 ) between group P1 and P2. Conclusion : There are differences in histological appearance of mice’s kidney i.e oedema of proximal tubules, between the groups which was not given Hedyotis corymbosa with the groups given, in accordance with the increasing doses . Keywords : Balb/c mice, Hedyotis corymbosa, proximal tubule