PENGARUH PEMBERIAN EKSTRAK HEDYOTIS CORYMBOSA DOSIS BERTINGKAT TERHADAP PRODUKSI NITRIC OXIDE MAKROFAG MENCIT BALB/C YANG DIINFEKSI DENGAN SALMONELLA TYPHIMURIUM

Abstract

Backgrounds: Typhoid fever can be found every year in Indonesia . Hedyotis corymbosa was proved to raise immune responses during intracell bacteria infection. Nitric oxide (NO) can be produced by T helper cell type 1. NO controlling the immune responses as effectors to kill bacteria by inducing apoptosis T cell type 1. This was possible if the macrophage be activated. Objective: To prove the administering of Hedyotis corymbosa effecting the NO production in peritoneal macrophage of Balb/c mice which inffected Sallmonella typhimurium Method: This research was conducted experimentally ( post test only control group design ) to 30 Balb/c mices that become the sample.The sample was divided into 6 groups: K1 (administered standard diet), K2 (inffected by Sallmonela typhimurium), K3 ( administered Hedyotis corymbosa 80 mg), the treatment group which inffected Sallmonela typhimurium and administered Hedyotis corymbosa with every dose , P1 24 mg, P2 80 mg, P3 240 mg. After 14 days those mice were killed, then macrophage isolation from peritoneum cavity was conducted. Then, the NO production was calculated by the calibration curve for NO determination using Griess reaction. Results: There is a significant differences between K2 andP2(p=0.008), P1 and P2(p=0.047),P2 and P3 (0.008), but there is no a significant differences between P1 and P3(p=0.278) Conclusion: There are significant increases to NO production in P1 and P2 but there is a decrease NO production in P3 . Keywords: Hedyotis corymbosa, NO production, Sallmonela typhimurium