Examination of the Ratios of Inoculums of Buffalo Faeces and Buffer Solution on the In Vtro Fibrous Feed Digestibility

Abstract

An investigation has been conducted to examine the ratios of inoculums made of buffalo faeces def-ecated and buffer (artificial saliva) on the analysis of in-vitro digestibility of dry matter and organic matter of rice straw, corn forage, elephant grass, pangola grass, and peanut straw, respectively. The ratios between feces to buffer provided were 1:4, 1:2, 2:3, 2:1, and 1:5. Rumen inoculums were obtained from two mature female rumen-fistulated buffaloes maintaining with mixed ration of 70% elephant grass and 30% concen-trates fed ad libitum (3% dry matter requirements to body weight). Rumen fluid was collected before morning feeding. After 10-days acclimatization, the specified data were collected. The results showed that inoculums ratios of 1 :2 were significantly affected the in-vitro digestibility of forages tested. The efficacity of this test was 95%. Therefore, faeces and buffer ratios were potential to be used for inoculums for in-vitro feed digestibility assay during 48 hours incubation period. Keywords  : buffalo, inoculum, faeces, buffer, rumen  fluid, in  vitro