Pengaruh Pemberian Vitamin C Terhadap Kadar Kolesterol Total Serum Tikus Wistar Jantan Hiperlipidemia Setelah Perlakuan Jus Lidah Buaya ( Aloe vera Linn )

Abstract

Latar Belakang: Hiperlipidemia sebagai salah satu faktor terjadinya penyakit degeratif meningkat dewasa ini. Aloe vera mampu menurunkan kadar kolesterol total serum penderita hiperlipidemia, karena mengandung asam nikotinat, vitamin C, asam folat, selenium, magnesium, enzim lipase, lignin dan anthraquinon. Selain itu pemberian vitamin C yang merupakan antioksidan diharapkan meningkatkan efek tersebut. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian vitamin C terhadap kadar kolesterol total serum tikus hiperlipidemia yang telah diberi jus Aloe vera. Metoda: Penelitian dengan desain Pre and Post Randomized Controlled Group Design dilaksanakan terhadap tikus Wistar yang telah dibuat hiperlipidemia. Tikus diberi jus Aloe vera dengan dosis 4 ml/hari selama 15 hari. Kemudian dilanjutkan pemberian vitamin C selama 3 hari dengan dosis 3,38 mg dan 11,25mg. Dilakukan pemeriksaan kadar kolesterol total serum menggunakan metode CHOD-PAP. Data yang diperoleh dianalisis menggunakan uji t berpasangan dan anova pada derajat kemaknaan 5%. Hasil: Pemberian jus Aloe vera terbukti mampu menurunkan kadar kolesterol total serum secara bermakna (p=0,000). Pemberian vitamin C pada kelompok perlakuan yang telah diberi jus aloe vera juga terbukti lebih menurunkan kadar kolesterol total secara bermakna (p=0,000). Simpulan: Pemberian vitamin C secara bermakna menurunkan kadar kolesterol total serum pada tikus hiperlepidemia yang telah diberi jus Aloe vera. Selain itu peningkatan dosis vitamin C berpengaruh secara bermakna dalam menurunkan kadar kolesterol total serum tikus. Kata kunci: Aloe vera, Vitamin C, kolesterol total serum, hiperlipidemia The Effect of Orally Administered Vitamin C on The Total Cholesterol Serum Level in Hiperlipidemic Wistar Rats Which Have Been Given Aloe vera Linn. Juice Before Endang Sri Sunarsih* and Slamet Pamuji* * Background: Hyperlipidemia as one of the factor of degerative disease has been increased recently. Aloe vera was supposed to decrease level of total cholesterol serum, because it contains of nikotinic acid, vitamin C, folic acid, selenium, magnesium, lipase enzyme, lignin and anthraquinon. The administration of vitamin C also suspects to increase the effect. Thus, a study determines the effect administration of vitamin C for total cholesterol serum level of the rats which have been given Aloe vera before. Methode: An experimental study using control group with pre and post test design was carried out to already make hyperlipidemic Wistar rats. They were given diet containing 4 ml Aloe Vera juice per day for 15 days. Then will be continued by administration of vitamin C 3,38mg and 11,25 mg doses for 3 days. Total cholesterol serum level was determined using CHOD-PAP method. Data were analyzed using paired t-test and one way ANOVA. The degree of significant was 5%. Result: The administration of Aloe vera juice were significantly (p=0.000) decreased the total cholesterol serum level of hiperpidemic rats. The administration of vitamin C also were significantly (p=0.000) more decreased the total cholesterol serum level which have been given Aloe vera juice before. Conclusion: The administration of vitamin C significantly reduced the total cholesterol serum level in hyperlipidemic rats which have been given Aloe vera juice before. Vitamin C doses in high dose also proved a significant effect in decrease totally cholesterol serum level, better than the lower. Keyword: Aloe vera, Vitamin C, total cholesterol serum, hyperlipidemic.