PENGARUH EKSTRAK Andrographis paniculata (SAMBILOTO) TERHADAP KADAR SERUM GLUTAMAT PIRUVAT TRANSAMINASE (SGPT) TIKUS WISTAR YANG DIBERI PARASETAMOL

Abstract

The Effect of Andrographis paniculata Extract (Sambiloto) to The Serum Glutamat Piruvat Transaminase (SGPT) Level in Wistar Rats Administered with Paracetamol Andi Dian Astykasary* , dr.M.Masjhoer** Background : A research obout Andrographis paniculata (sambiloto) to the mice which were induced by BHC (hepatotoxic agent) showed that sambiloto could incresed the activity of antioxidant enzymes, increased the level of glutathion and decreased the activity of lipid peroxidase enzymes. Paracetamol hepatotoxicity is dose dependend. In very high dose, the toxic metabolit of paracetamol can caused liver cell injury so that the intracellular enzymes such as Glutamat Piruvat Transaminase (GPT) released. The objective of this experiment was to find out the effect of sambiloto’s extract to SGPT level in Wistar rats administered with paracetamol. Methode : The experimental study with “the post test only control group design”, used 20 wistar male rats which were divided into 4 groups. Group K1 received only standard diet. Group K2 received paracetamol 1350mg/kgBW on the 8th day. Group K3 received sambiloto’s extract on the 1st-7th day and received paracetamol on the 8th day. Group K4 received paracetamol on the 8th day and sambiloto’s extract on the 8th-10th day. All groaups were terminated on the 10th day and the SGPT level were examinated. Data were analized by Anova and Bonferroni. Result : The outcome of Anova test showed significant differences between all groups (p=0,000). Bonferroni test of differentiation between all groups was significant (p=0,000) . Conclusion : Sambiloto’s extract decreased SGPT level in Wistar rats administered with paracetamol. Keywords : sambiloto, liver, Serum Glutamat Piruvat Transaminase, paracetamol Pengaruh Ekstrak Andrographis paniculata (Sambiloto) Terhadap Kadar Serum Glutamat Piruvat Transaminase (SGPT) Tikus Wistar yang Diberi Parasetamol Andi Dian Astykasary* , dr.M.Masjhoer** Latar Belakang: Dari penelitian tentang Andrographis paniculata (sambiloto) terhadap mencit yang diinduksi BHC (suatu zat hepatotoksik) didapatkan bahwa sambiloto dapat meningkatkan aktivitas enzim antioksidan, meningkatkan pengisian glutathion dan menurunkan aktivitas enzim lipid peroksidase. Hepatotoksisitas parasetamol tergantung pada besarnya dosis. Dalam dosis yang sangat tinggi parsetamol dapat menyebabkan kerusakan sel hepar sehingga mengakibatkan dilepaskannya beberapa enzim intrasel antara lain, Glutamat Piruvat Transaminase (GPT). Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak sambiloto terhadap kadar SGPT tikus Wistar yang diberi parasetamol. Metode : Penelitian eksperimental dengan rancangan “the post test only control group design”, menggunakan sampel 20 ekor tikus Wistar jantan dibagi menjadi 4 kelompok. Kelompok K1 hanya diberi pakan standar. Kelompok K2 diberi parasetamol 1350mg/kgBB pada hari ke 8. Kelompok K3 diberi ekstrak sambiloto 500mg/kgBB pada hari 1-7 dan diberi parasetamol pada hari ke-8. Kelompok K4 diberi parasetamol pada hari ke-8 dan ekstrak sambiloto pada hari ke 8-10. Pada hari ke-10 dilakukan terminasi dan diperiksa kadar SGPT. Data dianalisis dengan uji Anova dan Bonferroni. Hasil : Hasil uji Anova menunjukkan perbedaan yang bermakna pada seluruh kelompok (p=0,000). Dari uji Bonferroni didapatkan perbedaan yang bermakna pada seluruh kelompok (p=0,000). Kesimpulan : sambiloto mampu menurunkan kadar SGPT tikus Wistar yang diberi parasetamol. Kata kunci : sambiloto, Serum Glutamat Piruvat Transaminase, hepar, parasetamol.