PENGARUH PEMBERIAN VITAMIN E TERHADAP MOTILITAS SPERMATOZOA MENCIT JANTAN STRAIN BALB/C YANG DIBERI PAPARAN ASAP ROKOK

Anggraini, Diana (2006) PENGARUH PEMBERIAN VITAMIN E TERHADAP MOTILITAS SPERMATOZOA MENCIT JANTAN STRAIN BALB/C YANG DIBERI PAPARAN ASAP ROKOK. Undergraduate thesis, Faculty of Medicine.

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Abstract

The effect of Vitamin E to Sperm Motility of Male Balb/C mice exposed by Cigarette Smoke Diana Anggraini*, Ahmad Zulfa Juniarto** Background: Cigarette smoke contains ± 4000 component such as alkaloid, nitrosamine, anorganic molecul, and ROS subtance, that influence man infertility. Vitamin E as antioxidant estimated could protect sperm from ROS. The aim of this research is to prove the effect of vitamin E on sperm motility of male Balb/C exposed by cigarette smoke. Methode: This was an experimental study with a post test only control group design with 30 male Balb/C mice as a subject, which was divided into 6 groups: K- was not treated; K+ was exposed by cigarette smoke; P1; P2; P3; P4 were exposed by cigarette smoke and 0,02 mg/g bw; 0,04 mg/g bw; 0,06 mg/g bw; 0,12 mg/g bw vitamin E. Treatment is given in 14 days, at 15th day the mice was terminated, then the sperm motility was examined with straight quick move and slow move criteria and then analyzed with SPSS 13.00 Windows programm. Result: The mean motility of sperm in the K- group was 45,23+18,18; K+ was 13,50+16,89; P1 was 48,19+23,61; P2 was 31,40+31,00 and 65,57+12,77 at the P3 group, while the P4 was died. Kruskal-Wallis test between all groups, Mann-Whitney test among K- with K+, K+ with P1 and P3 were significantly different. Conclusions :Vitamin E supplementation maintains sperm motility of male Balb/c mice exposed by cigarette smoke. Keywords : vitamin E, sperm motility, cigarette smoke, male Balb/C mice Pengaruh Pemberian Vitamin E Terhadap Motilitas Spermatozoa Mencit Jantan Strain Balb/C Yang Diberi Paparan Asap Rokok Diana Anggraini*, Ahmad Zulfa Juniarto** Latar Belakang: Asap rokok mengandung ± 4000 komponen seperti alkaloid, nitrosamine, molekul anorganik, dan substansi berupa SOR yang dapat mempengaruhi fertilitas pria. Vitamin E sebagai antioksidan diperkirakan dapat melindungi spermatozoa dari SOR. Tujuan penelitian ini adalah membuktikan pengaruh vitamin E terhadap motilitas spermatozoa mencit Balb/c jantan yang diberi paparan asap rokok. Metodologi: Penelitian eksperimental laboratoris dengan pendekatan post test only control group design. Subyeknya adalah 30 ekor mencit jantan strain Balb/c yang dibagi secara acak menjadi enam kelompok. Kelompok: K- tidak mendapat perlakuan; K+ diberi paparan asap rokok; P1; P2; P3; P4 diberi paparan asap rokok dan vitamin E dosis 0,02 mg/g BB; 0,04 mg/g BB; 0,06 mg/g BB; 0,12 mg/g BB. Perlakuan berlangsung selama 14 hari, pada hari ke15 mencit diterminasi, kemudian dilakukan pemeriksaan motilitas spermatozoa dengan kriteria bergerak cepat lurus dan bergerak lambat yang diolah dengan program SPSS 13.00 for Windows. Hasil: Rerata motilitas spermatozoa pada K- adalah 45,23+18,18; K+: 13,50+16,89 ; P1: 48,19+23,61; P2: 31,40+31,00 dan 65,57+12,7 pada P3, sedangkan P4 sampel mati semua. Perbandingan pada semua kelompok dan antara dua kelompok (K- dengan K+, K+ dengan P1 dan P3) terdapat perbedaan yang bermakna (p<0,05). Kesimpulan: Pemberian vitamin E dapat mempertahankan motilitas spermatozoa mencit jantan strain Balb/c yang diberi paparan asap rokok. Kata kunci: vitamin E, motilitas spermatozoa, asap rokok, mencit jantan strain Balb/C

Item Type:Thesis (Undergraduate)
Subjects:R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions:Faculty of Medicine > Department of Medicine
Faculty of Medicine > Department of Medicine
ID Code:20234
Deposited By:Ms Lutfiatun Heni
Deposited On:12 Aug 2010 13:23
Last Modified:12 Aug 2010 13:23

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