PENGARUH KOPI TERHADAP KADAR ASAM URAT DARAH Studi Eksperimen Pada Tikus Rattus Norwegicus Galur Wistar The Influence of Coffee on the Blood Uric Acid Level An Experiment Study Rattus Norwegicus Wistar Strain Rat

Abstract

Background : Coffee is one of the several favorite drink in the world. There is still a controversial effect of coffee, whether coffee could increase or decrease the blood uric acid level. Study about the influence of coffee on the blood uric acid level has never been conducted in Indonesia. Objective : To prove that coffee could decrease blood uric acid level in the hyperuricemia Rattus Norwegicus Wistar Strain Rat. Method : A pre and post test design that has been done on 24 wistar strain rats that were divided randomly into 4 groups. On the 1st week and 2nd week all groups were fed high purin diet. On the 2nd week all group were given coffee,too. Group 1 were given 0.36 ml coffee solution/ day and the other group were given 0.72 ml/ day; 1.14 ml/ day and 2.16 ml/ day. Blood were drawn at the beginning before high purin diet were given, before and after coffee treatment. The blood uric level were examined using spectrophotometry. Data were analyzed using Pair- t test and One Way Anova. Result : There were decreased blood uric acid level in the group receiving 0,72 ml /day and 2,16 ml coffee solution/day. Conclusion : Coffee is able to reduce blood uric acid level in experimental rats. Latar Belakang : Kopi merupakan salah satu minuman kegemaran masyarakat dunia. Masih ada kontroversial dari efek kopi dimana kopi dapat meningkatkan atau menurunkan kadar asam urat. Penelitian tentang pengaruh kopi terhadap kadar asam urat darah belum pernah dilakukan di Indonesia. Tujuan : Membuktikan bahwa kopi mampu menurunkan kadar asam urat serum darah pada tikus Wistar dengan hiperurikemia Metode : Suatu studi eksperimen pre and post test design dilakukan pada 24 tikus Wistar yang secara random dibagi menjadi 4 kelompok. Pada minggu pertama dan minggu ke-2 semua kelompok diberi pakan tinggi purin. Pada minggu ke-2 semua kelompok juga diberikan larutan kopi. Pada kelompok perlakuan 1 diberikan larutan kopi 0,36 ml/hari, sedangkan kelompok lainnya diberikan : 0,72 ml/hari ; 1,14 ml/hari dan 2,16 ml/hari. Kadar asam urat darah diukur sebelum pemberian pakan tinggi purin, sebelum serta setelah pemberian larutan kopi. Kadar asam urat darah diperiksa dengan menggunakan spektrofotometri. Analisis data menggunakan uji t-berpasangan dan One Way Anova. Hasil : Ada penurunan kadar asam urat setelah perlakuan pada kelompok yang memperoleh larutan kopi 0,72ml/hari dan 2,16 ml/hari. Kesimpulan : Larutan kopi dapat menurunkan kadar asam urat pada tikus percobaan.