Genetics Characterization of Aceh Cattle Utilizing Microsatellite DNA Analyses

Abstract

The aims of this study were to describe the variation of microsatellite alleles of Aceh cattle. The blood samples (160 samples) were collected from Aceh Besar, Pidie, North Aceh district and Banda Aceh city. For the outgroup comparison, the blood samples were collected from 10 Bali cattle, two samples of each collected from Madura, PO and Pesisir cattle. Sixteen markers were used for genotyping microsatellite DNA. The molecular data were analyzed using Minitab 14.13, Mega4 and Arlequin 3.11 and Excel software. The result of microsatellite analyses showed that the averages allele number per locus was 10,25 ± 2,07. The percentage of the heterozygosity of Aceh cattle was higher than those of Bali Cattle, but lower compared to those of Madura, PO and Pesisir cattle. Based on the microsatellite alleles analyses, the Aceh cattle were in the same cluster as PO cattle and were in the same branch of the phylogeny tree as Pesisir and Madura cattle. Keywords: Aceh cattle, Allele, DNA, Microsatellite