The Viability and Membrane Integrity of Spotted Buffalo Epididymal Sperm in Addition of Maltose into AndromedExtender

Herdis, Herdis and Surachman, M and Yulnawati, Yulnawati and Rizal, M and Maheshwari, H (2008) The Viability and Membrane Integrity of Spotted Buffalo Epididymal Sperm in Addition of Maltose into AndromedExtender. Journal of the Indonesian Tropical Animal Agriculture, 33 (2). pp. 101-106. ISSN 0410-6320

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Abstract

The aim of this study was to investigate the viability and membrane integrity of spotted buffalo epididiymal sperm. Andromed was used as control extender and the combination of Andromed with 0.2% (P1) and 0.4 % (P2) maltose were used for the treatments. The results showed that the percentage of motility after thawing in P1 media (46.00  2.00 %) was not significantly different (P>0.05) from P2 (44.00  2.00 %), but there was significantly different (P<0.05) from K (41.00  2.00 %). The percentage of live sperm after thawing in P1 (59.20  1.17 %) was not significantly different (P>0.05) from P2 (60.60  1.62 %), but significantly different (P<0.05) from K (52.20  2.48 %). There was no significantly different (P>0.05) in the percentage of membrane integrity of spotted buffalo epididymal sperm at the three stages of cryopreservation process in the three different extenders. In conclusion, the addition of maltose into Andromed could maintain the viability and membrane integrity of spotted buffalo epididymal sperm after thawing. Keywords: Viability, Plasma membrane, Epididymal sperm, Maltose, Spotted buffalo

Item Type:Article
Subjects:S Agriculture > S Agriculture (General)
Divisions:Faculty of Animal and Agricultural Sciences > Department of Animal Agriculture
ID Code:18998
Deposited By:Mr. Nutrisi FP Admin
Deposited On:05 Aug 2010 20:44
Last Modified:05 Aug 2010 20:44

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