EFEK CYCLOPHOSPHAMID-TRANSFER FACTOR TERHADAP PROLIFERASI SEL ( AgNOR ) DAN VOLUME TUMOR ADENO CA MAMMAE MENCIT The effects of cyclophosphamid-transfer factor on cells proliferation (AgNOR) and volume of mice adeno ca mammae tumor

Abstract

Background : cancer patients have decreased immune system due to suppression by cancer cells and systemic therapy. Transfer factor is oligoribonukleotida that improve the memory function of T cell and the amount of NK cell. It still controversial whether TF has capability in reducing cells proliferation and the tumor volume. Purposes : to analyze the effects of cyclophosphamid-transfer factor on cells proliferation and the volume of mice adeno Ca mammae tumor. Methods : experimental laboratory research has been done by “ post test only control group design “ .Twenty female pure C3H mice were devided into four groups: K as a control group, P2 given the cyclophosphamid, P3 given the transfer factor and P4 given the cyclophosphamid – transfer factor combination. As a dependent variable were cells proliferation ( AgNOR ) and tumor volume. ANOVA test was used to analyze the differences of cells proliferation (AgNOR ) and tumor volume among the four groups with 0,05 significance level. Results : The difference of cells proliferation ( AgNOR) was p=< 0,001. There were differences among the four groups. The difference of tumor volume was p=0,086. There were no differences among the four groups. Correlation number between cells proliferation (AgNOR ) and tumor volume was 0,496 value with the significant level 0,026. There is a substansial correlation between cells proliferation ( AgNOR ) and tumor volume. The influence of cells proliferation (AgNOR ) to tumor volume was 20,4% and 79,6% tumor volume was influenced by other factors that hasn’t been conducted in this research. Conclusion : cyclophosphamid-transfer factor has a synergistic effect to the cells proliferation tumor Latar belakang : penderita kanker mengalami penurunan respon imun akibat supresi sistem imun oleh sel kanker dan modalitas terapi sistemik. Transfer factor merupakan oligoribonukleotida yang mampu meningkatkan fungsi memori sel imun dan meningkatkan jumlah sel NK. Apakah TF dapat menghambat proliferasi sel dan volume tumor masih belum dapat dibuktikan. Tujuan : Untuk menganalisa efek pemberian cyclophosphamid-transfer factor terhadap proliferasi sel dan volume tumor Adeno Ca mamae mencit Metode : Penelitian eksperimental laboratorik dengan desain “Post test only control group”. Menggunakan mencit betina C3H murni berjumlah 20 ekor. Sampel dibagi 4 kelompok: K sebagai kontrol, P1 diberi cyclphosphamid, P2 diberi transfer factor dan P3 diberi kombinasi cyclophosphamid-transfer factor. Sebagai variabel tergantung adalah proliferasi sel ( AgNOR) dan volume tumor. Analisis perbedaan proliferasi sel ( AgNOR ) dan volume tumor antara keempat kelompok menggunakan uji ANOVA dengan tingkat kepercayaan 95 %. Hasil : Perbedaan proliferasi sel (AgNOR) diperoleh nilai p=<0,001. Terdapat perbedaan bermakna antara keempat kelompok variabel. Perbedaan volume tumor didapatkan nilai p=0,086, sehingga tidak didapatkan perbedaan antara keempat kelompok. Angka korelasi antara proliferasi sel (AgNOR) dan volume tumor didapatkan nilai 0,496 dengan nilai signifikansi 0,026. Ini berarti hubungan antara proliferasi sel (AgNOR ) dan volume tumor bersifat substansial. Pengaruh proliferasi sel (AgNOR) terhadap volume tumor sebesar 20,4%, 79,6% volume tumor dipengaruhi oleh faktor lain yang tidak diteliti dalam penelitian ini. Kesimpulan : cyclophosphamid-transfer factor mempunyai efek sinergis dalam menurunkan proliferasi sel tumor.