PERBEDAAN KADAR RESISTIN ANTARA PENDERITA DENGAN DAN TANPA PENYAKIT JANTUNG KORONER THE DIFFERENCE OF RESISTIN LEVELS BETWEEN PATIENT WITH AND WITHOUT CORONARY HEART DISEASE

Abstract

Background : Resistin, a novel plasma protein, belongs to a family of cysteine-rich secreted polypeptides that are mainly produced by monocytes/macrophages in human. Resistin linked to insulin resistance and obesity in rodents which is identified in atheromas in human, has been shown to play a potential role in atherosclerosis. Resistin levels were reported to increase in coronary heart disease (CHD), while data concerning resistin in Indonesian people are lacking. This study examined the difference of resistin levels between CHD and without CHD patients. Aims of Study : To prove that serum resistin levels in CHD patients were higher than in the non CHD patients. Design and Methods : Cross sectional study of 40 blood specimens from 40 patients based on the presence of coronary artery stenosis were enrolled and divided into two groups, CHD and non CHD patients. Serum resistin levels were determined by enzyme linked immunosorbent assay (ELISA) method. Data were analyzed with Mann-Whitney test. Results : Serum resistin levels were higher in patients with CHD than in those without CHD patients (14.00 and 9.82 ng/ml, respectively); p<0.05. Serum resistin levels were also significantly different between CHD [ Median resistin, n=20 : 14.00 (5.70-40.08) ng/ml ] and non CHD patients, [ n=20 : 9.82 (4.12-15.90) ng/ml ]; p=0.02. Conclusions : Serum resistin levels were significantly higher in patients with CHD than in those without CHD patients. Suggestions : Further studies are needed to define whether resistin can reflect the severity of coronary atherosclerosis and the role of resistin in development of CHD. Latar Belakang : Resistin, suatu protein plasma baru, termasuk dalam keluarga polipeptida yang mensekresi sistein dan diproduksi oleh monosit/makrofag pada manusia. Resistin berhubungan dengan resistensi insulin dan obesitas pada tikus, juga didapatkan di ateroma pada manusia, menunjukkan resistin berperan potensial dalam aterosklerosis. Kadar resistin telah dilaporkan meningkat pada penyakit jantung koroner (PJK), sementara data mengenai resistin pada orang Indonesia masih kurang. Penelitian ini membandingkan kadar resistin antara penderita PJK dan tanpa PJK. Tujuan Penelitian : Membuktikan bahwa kadar serum resistin pada pasien PJK lebih tinggi dibandingkan tanpa PJK. Disain dan Metoda : Pendekatan belah lintang dengan 40 spesimen darah dari 40 pasien, berdasarkan adanya stenosis arteri koroner dibagi menjadi 2 kelompok, PJK dan tanpa PJK. Kadar serum resistin diukur dengan metode enzyme linked immunosorbent assay (ELISA). Data dianalisis dengan uji Mann-Whitney. Hasil : Kadar serum resistin lebih tinggi pada penderita PJK dibandingkan tanpa PJK (14,00 dan 9,82 ng/ml, secara berurutan); p<0,05. Kadar serum resistin juga berbeda bermakna antara penderita PJK [Median resistin, n=20 : 14,00 (5,70-40,08) ng/ml] dan tanpa PJK, [ n=20 : 9,82 (4,12-15,9) ng/ml ]; p=0,02. Kesimpulan : Kadar serum resistin pada penderita PJK lebih tinggi secara bermakna dibandingkan tanpa PJK. Saran : Perlunya penelitian lebih lanjut untuk mengetahui apakah resistin dapat mencerminkan beratnya aterosklerosis koroner dan peran resistin dalam perkembangan PJK.