COMPARlSON OF SHORT AND LONG WAVELENGTH OF NEAR INFRARED SPECTROSCOPY FOR PREDICTING NITROGEN AND ALLAKTOIN IN URINE

Abstract

ABSTRACT Total 130 urine samples collected from d.J.iry on[e were studied for knowing the possibility and accuracy of the use of long and short wavelength of near infrared spectroscopy (NIRS) for nitrogen and allantoin content in urine. The sJ.mples were randomly separated for the calibration (n=9~) and the validation samples (n=36). Short and long wavelength of NIRS was selected in the range of 700-1350 and 1350-2500 run, respectively. Four wavelengths selected from short wavelength were found mainly related to the N-H, C-H or O-H structure in both for nitrogen at 1225, 970, 1060 and 806 run and for all.wtoin at 990, 1030, 1170 and 815 run, respectively. M~while, the selection in long w:lvelcngth was found rn:l.inly related to the N-H bound or protein for e;:lch component at 1430, 1530, 2242 and 1960 run for nitrogen and 2180, 2242, 1978 and 1530 run for allantoin, respectively. CoeffiCient correlation (and standard error) of prediction \\ith calibration equations using short wavelength were 0.85 (0.19), and 0.85 (47.7) for nitrogen and allantoin, respectively. Those values obwined from long wavelength were found to be 0.91 (0.13) and 0.92 (42.3), respectively. Judgement of validation ",ith RPD values (the ratio of stand:Hd deviation of reference data in prediction sJ.mple to the standard error of prediction by r\IR) for nitrogen and allantoin predicted by short wavelength were found 2.06 and 2.42, respectively. These values were lower than 2.5 the limit value recommended available for application. However, the use of long wavelength of r\IRS showed an possibility to predict nitrogen and allantoin in urine as shO\\TI by high RPD values to be 2.94 and 2.70, respectively. Key words: Near infrared, AIIJr,toin, Urine.