POTENCY OF LIGNOCELL LOSE DEGRADING BACTERIA ISOLATED FROM B FFALO AND HORSE GASTROINTESTINAL TRACT AND ELEPHANT D NG FOR FEED FIBER DEGRADATION

Abstract

Lignin is limiting factor for cellulose and hemicellulose degradation in rumen.Isolation and selection bacteria from buffal and horse gastr intestinal tract and elephant dung could be found bacteria that have superi rity t degrade lignin,xylan,and cellul se.Those animals were chosen because they were herbivores that consume low quality crude fiber as their main energy sources. Lignocellulose degrading bacteria were is lated by Hungate selective media,by using lignin (tannic acid),xylan,and cellulose as selective substrates.The m rphological identification used an enrichment media by measuring col r,colony size,diffusion zone,clear zone,and biochemical identification using production of ligninase,xylanase,and cellulase enzymes.The best lignocellulose degrading bacteria then was determined by the m rphological and biochemical character.This study showed that lignocellulose degrading bacteria could be found in gastr intestinal tract of buffal and horse,and elephant dung.Highest number colony was found in samples fr m buffalo's c lon (376),f llowed by horse's cecum (203),elephant ’s dung (46),buffalo ’s cecum (23),buffalo's rumen (9)and horse ’s colon (7).The highest is lates activity f lignolytic,xylanolytic,and cellulolytic were reached by buffalo ’s cecum (7.64),horse's cecum (6.27),and buffalo ’s colon (2.48).Meanwhile the highest enzymes productivities were:buffalo ’s cecum (0.0400 µm l),horse ’s cecum (1.3912 µmol)and buffalo ’s colon (0.1971 µmol).Based on morphologycal character and bi chemical test,it could be concluded that lignolytic fr m buffalo ’s cecum,xylanolytic fr m horse ’s cecum,and cellul lytic fr m buffalo ’s colon were the superior isolates and they were 99%analyzed as Enterococcus casseliflavus/gallinarum species. Keywords :bacteria,buffalo,degradation,elephant,feed fiber,horse,lignocellulose.