THE EFFECT OF CLOVE STEM OIL (Oleum caryophylli )ON THE GROWTH OF ESCHERICHIA COLI ISOLATED FROM NATIVE CHICKEN,CATTLE AND PIG

Abstract

The objective of thi tudy wa to examine the effect of Clove Stem Oil (Oleum caryophylli )or CSO extract on the growth of Escherichia coli .A 4 x 3 Factorial De ign wa applied in thi tudy.The fir t factor wa 4 type of E.coli i olate that collected from native chicken (C),young cattle (A),piglet (B1)and pig (B2),while the econd factor wa 3 concentrations of CSO extract,that wa 50.00,25.00 and 12.50 percent .Fifteen micro liter of CSO extract wa dropped in terile paper di ks.The e di k were laid on the MEU agar media previou ly inoculated with each of the four i olate and were incubated overnight at 37 0 C.The bacterial growth inhibition zone were observed and mea ured.The re ult demon trated that the higher concentration of the CSO extract ,the higher bacterial growth inhibition effect obtained.Fhe growth inhibition effect of the CSO extract on E.coli i olate C,A,B1 and B2 were ignificantly different at P<0.05.It could be concluded that the CSO extract were effective to control the E.coli growth. Keywords :Clove Stem Oil (Oleum caryophylli ),traditional medicine,native chicken,cattle,pig.