PENGARUH PEMBERIAN MINYAK BUAH MERAH TERHADAP KADAR ALT DAN TNF-α SERUM TIKUS SPRAGUE DAWLEY YANG MENGALAMI KERUSAKAN HEPAR KARENA INDUKSI CCl4 THE EFFECTS OF RED FRUIT OIL ON ALT AND TNF- α SERUM LEVELS OF INJURED LIVER SPRAGUE DAWLEY RAT INDUCED BY CCl4

Abstract

Latar Belakang : Minyak buah merah (MBM) kaya β−karoten dan α−tokoferol yang merupakan antioksidan pemutus rantai. Sampai saat ini penelitian tentang pengaruh MBM terhadap tubuh masih sedikit, termasuk pemakaiannya untuk mengobati penyakit hepar masih mengundang pro dan kontra di kalangan kesehatan. Tujuan Penelitian : Membuktikan adanya pengaruh pemberian MBM terhadap kadar ALT dan TNF-α serum tikus Sprague Dawley yang mengalami kerusakan hepar karena induksi CCl4. Desain dan Metoda : Penelitian eksperimental laboratorik menggunakan randomized pretest postest control group design. Tikus Sprague Dawley jantan 24 ekor dibagi menjadi 4 kelompok yaitu KN (kontrol negatif), KP (kontrol positif), kelompok P1 diinduksi CCl4 kemudian diberi MBM, kelompok P2 diberi MBM selanjutnya diinduksi CCl4. Diperiksa kadar ALT dan TNF-α serum. Uji beda menggunakan uji non parametrik Kruskal Wallis dan Mann Whitney. Hasil : δ kadar ALT ± SD (IU/l) KN:-0,53±7,19, KP:-18,7±8,33, P1:-19,72±11,45, P2:104,67±74,26. Uji Kruskal Wallis mendapatkan p<0,001. Uji Mann Whitney antar kelompok mendapatkan p< 0,05 kecuali antara KP dengan P1. δ kadar TNF-α serum (pg/ml) KN:-12,02±22,64, KP:8,50±28,23, P1:6,72±15,91, P2: 14,53±11,12. Uji Kruskal Wallis mendapatkan p > 0,05. Simpulan: MBM tidak menurunkan kadar ALT dan TNF-α serum pada kerusakan hepar karena induksi CCl4 namun menghambat peningkatan kadar ALT apabila diberikan sebelum induksi CCl4. Saran : Penelitian lanjut pengaruh pemberian MBM pada pemakaian jangka panjang Background: Red fruit oil (MBM) is known to contain β carotene and α tocoferol that has been suggested as breaking chain antioxidant. There has been few studies investigating the effects of MBM on human organs and its usage for treating injured liver is a subject of controversy among medical practitioners. Aims: To prove the effect of MBM administration on ALT and TNF-α serum levels of injured liver Sprague Dawley rat induced by CCl4. Design and Method: An experimental study using randomized pretest post test control group. Twenty four male Sprague Dawley rats were divided into four groups including negative control (KN), positive control (KP), group treated CCl4 prior to the red fruit administration(P1) and group treated with red fruit oil prior to CCl4 administration(P2).The serum levels of ALT and TNF-α were assessed. Non parametric test of Kruskal Wallis and Mann Whitney was applied. Results : δ of serum levels of ALT for the four groups were 0.53 ±7.19 ; 18.7 ± 8.33; 19.72 ± 11.45; 104.67 ± 74.26 respectively. Kruskal Wallis test resulted in p< 0, 001. Mann Whitney test resulted in p < 0, 05 except between KP and P1. δ of serum levels of TNF-α for the four groups were 12.02 ± 22.64; 8.50 ± 8.23; 6.72 ± 15.91; 14.53 ± 11.12 respectively. Kruskal Wallis test resulted in p> 0, 05. Conclusion: MBM administration does not lower the serum level of ALT and TNF-α in the CCl4-induced hepatotoxicity, but inhibits the increase of the levels of ALT when it is given before CCl4 induction. Suggestion: More studies are needed to find out the effects of long term usage of MBM.