STUDI PEMBUATAN Zn-FITAT DAN DEGRADASINYA DI DALAM CAIRAN RUMEN [Study on Zn-Fitat Processing and Its Degradation In Rumen Fluid]

Abstract

ABSTRAK Asam fitat banyak terdapat dalam biji-bijian. Senyawa ini dapat mengikat kuat Zn. Penelitian ini mempelajari pembuatan Zn-fitat dan degradasinya di dalam cairan rumen. Asam fitat diekstraksi dari pollard menggunakan larutan asam cuka 1% dengan rasio 1:3. Ekstrak asam fitat direaksikan dengan larutan ZnCl2 pada perbandingan molaritas 1:2. Karakterisitik Zn-fitat ditentukan dengan mengukur bahan kering dan kadar mineral endapan. Produk Zn-fitat diinkubasikan dan diukur degradasinya dalam sistem rumen in vitro. Hasil menunjukkan bahwa pembentukan kompleks Zn-fitat dari ekstrak pollard dengan ZnCl2 menghasilkan endapan sebanyak 10,7 mg/L dengan kadar Zn yang terikat sebesar 29,87% serta turut mengendapkan Mg dan Na. Kompleks Zn-fitat didegradasi lambat dalam cairan rumen dan percepatan degradasi dimulai pada jam ke-8. Kata Kunci : asam fitat, Zn-fitat, degradasi cairan rumen ABSTRACT Phytic acid ishigh in cereals grain. This compound may have high affinity to bound Zn tighty. The present experiment was aimed to study the process of Zn-phytate and its degradation in rumen fluid. Phytic acid was extracted from pollard using acetic acids solution at concentration 1%. Solution of acetic acid at concentration of 1% was used to produce phytic acid from pollard at ratio of 1:3. Phytic acid solution was mixed with ZnCl2 solution at a molarity ratio of 1:2. Zn-phytate characteristics were identified by dry matter and minerals content in the sediment. In vitro studies were conducted to evaluate the degradation of Zn-phytate. The results indicated that the formation of Zn-phytate in the pollard extract mixed with ZnCl2 solution at molarities ratio of 1:2 was 10,7 mg/L. Total Zn bound the phytic acid was 29,87%. Magnesium and Na were also precipitated in the sediment. Zn-phytate complex was degraded slowly in rumen fluid. High rate of Znphytate degradation was likely commenced after 8 h of incubation. Keywords : phytic acid, Zn-phytate, degradation, rumen fluid