Hidrolisis Ampas Tebu Secara Enzimatis Menggunakan Trichoderma reesei

Abstract

Bagasse (bagas)is cellulose biomass which more difficult to degredation dan coverted material than the other material, like starch. Enzymatics hydrolysis is the most promising technology to converted biomass to sugar. The objective of this study was to investigate by laboratories how far bagasse can produce the monomer sugar and to know the optimum operation condition process of the hydrolysis of bagasse using Trichoderma ressei. Dependent variable in this research are pretreatment temperature 1100C , pretreatment time 40 minutes, pressure 1 atm, hydrolysis temperature 500C ,and bagasse 5 gr. Independent variable in this research are pH 4 and 5, enzyme-substrat ratio 1:1 and 1:1,75 which analyzed yield of glucose every 6 hr in 48 hr. Time of hydrolysis is the most influence variable. The best result achived at 36 hr time with a hydrolysis yield of 30.4%/ Hydrolysis yield equation model is y = -0,005x2 + 1,047x – 2,228.