Profil Kromatogram dan Aktivitas Antibakteri Ekstrak Etanol Rimpang Bengle (Zingiber cassumunar Roxb) Terhadap Bakteri Escherichia coli In Vitro

Abstract

Chromatogram profile and antibacterial activity of ethanol extract from bengle rhizome (Zingiber cassumunar Roxb.) to Escherichia coli in vitro Background: Bengle (Zingiber cassumunar roxb.) was a plant which had been used by society since long time ago as a traditional medicine. It was predicted that there were anti-bacteria substances in bengle, so it might substitutes the utilization of conventional antibiotics. The aims of this research was to explore the chromatogram and antibacterial activity etanol extract of Bengle Rhizome against Escherichia coli. Methods: The method of these research were descriptive and experimental study using the post test only control group design. Ethanol extract of Bengle rhizome was made by maceration method with ethanol as solvent. Chromatogram profile was using thin layer chromatography and antibacterial activity against escherichia coli was done using dilution method and than cultured in Mac Conkey agar media. Results: The results showed thad, thin layer chromatography shown 4 spot under UV light 254 nm wave leng with colour, blue violet, blue violet, brown yellow, brown yellow and Rf value 0.21; 0.34; 0.45; 0.55. No bacterial growth at etanol extract of Bengle rhizome 12.5% on Mueller Hinton Broth media, nor at consentration of 25% on Mac Conkey media. Conclusions: The were 4 compounds predictly included of flavonoid, steroid, volatile oil and terpenoid group. Etanol extract of Bengle rhizome had minimum consentration inhibitory 12.5% and minimum bactericide consentration 25% against Escherichia coli.