Pengaruh Polifenol Teh Hijau Terhadap Sistem Imun Penderita Karsinoma Nasofaring yang Mendapat Radioterapi Kajian jumlah monosit, limfosit serta produksi TNF-􀄮, IFN-􀈖 dan IL-2 ex vivo

Abstract

The effect of green tea polyphenols on the immunity of nasopharyngeal carcinoma patients treated with radiotherapy: A study on the number of monocytes and lymphocytes, and production of TNF-􀄮, IFN-􀈖 and IL-2 ex vivo Background: Radiotherapy in patients with nasopharyngeal carcinoma gives side effects, among other is the suppression of immunity system. Green tea polyphenols (GTP) has been proven to modulate immune system. The study aimed to analyze the capacity of antioxidant GTP in inhibiting the suppression effect of radiotherapy to the immune system using monocytes, lymphocytes, TNF-􀄮, IFN-􀈖 and IL-2 parameters. Methods: An experiment study using pre-post test control group design was conducted to 50 NPC patients in II, III and IV stages, received 200 cGy/day 33 times. The treatment group was 25 patients consumed GTP (2900 mg of decaffeinated extract of green tea, containing 986 mg EGCG and 1711 mg of other polyphenols) 2 hours before and 10 hours after RT. The 10 ml blood of cubiti vein was taken 5 to 7 days prior and 3-5 hours after the whole RT. Monocytes and lymphocytes were cultured with autologous whole blood and TNF-􀄮 secretion of monocytes having been induced with LPS, IFN-􀈖 and IL-2 by lymphocytes having been induced with PHA and LPS respectively, were measured using ELISA. Results: In the post RT, the control group, monocyte, lymphocyte, TNF-􀄮, IFN-􀈖 and IL-2 had decreased, and the treatment group, monocyte, lymphocyte, and TNF-􀄮 decrease, while IFN-􀈖 and IL-2 increase. The t-test transformation logarithm data of monocyte, lymphocyte, and TNF-􀄮 comparing the two groups, the result of monocyte and lymphocyte was significantly different (p<0.05), except TNF-􀄮. The Mann-Whitney test the delta data of the IFN-􀈖 and IL-2 were significantly different (p<0.05). Conclusions: GTP significantly inhibit the decrease of the number of monocyte and lymphocyte as well as the production of IFN-􀈖 and IL-2, except TNF-􀄮.