WIDAYATI, RETNO INDAR (2004) Korelasi derajad lesi dermatitis atopik terhadap koloni Staphylococcus aureus dan Asosiasi derajad lesi dermatitis atopik terhadap Staphylococcus aureus enterotoxin-B". Masters thesis, program Pascasarjana Universitas Diponegoro.
| PDF - Published Version 1845Kb |
Abstract
Staphylococcus aureus is one of the triggers that can affect the extent of Atopic Dermatitis (AD) both in the colony form and its toxin. The toxins produced by S.aureus are Staphylococcus enterotoxin-A (SE-A), SE-B, SE-C, SE-D, SE-E and TSST-1, and SE-B to the most influential toxin toward the stage of AD's lesion. The aim of this study is to reveal the correlation between the stage of lesion in AD toward S.aureus and the association of the stage of lesion in AD toward Staphylococcus enterotoxin-B (SE-B). The study was conducted employing cross-sectional methode in the time span of 4.5 months on 14 AD patients coming to the Dermatology and Venerology outpatients clinic in Dr.Kariadi General Hospital in Semarang. The assessment of AD was with Hanifin & Rajka, the stage of the lesion with the ADSI index and the colony with culture in blood agar media. Sample was obtained from AD'lesion with 1cm in diameter using sterile cotton, followed by making a culture in a blood agar media to obtain the quantity of the colony. One colony of S.aureus was treated to SE-B toxin examination using immunodifusion (Ouchterlony) methode. Out of the 14 AD patients were revealed no correlation found between the stage of lesion with quantity of S.aureus colony and no association found between the stage of lesion in AD with SE-B toxin S.arueus merupakan salah satu pencetus yang dapat mempengaruhi derajad lesi Dermatitis Atopi (DA) baik dalam bentuk koloni maupun toksinnya. Toksin yang dihasilkan oleh S.aureus adalah Staphylococcus enterotoxin-A (SE-A), SE-B, SE-C, SE-D, SE-E dan TSST-1, dimana SE-B merupakan toksin yang paling berpengaruh terhadap derajad lesi DA. Tujuan dari penelitian ini adalah untuk mengetahui korelasi derajad lesi dermatitis atopik terhadap jumlah koloni S.aureus dan asosiasi derajad lesi dermatitis atopik terhadap titer Staphylococcus enterotoxin-B (SE-B). Penelitian dilakukan dengan metode cross sectional selama 5 bulan pada 14 penderita DA di Poliklinik PO Kulit dan Kelamin RSVP Dr Kariadi Semarang dan Puskesmas di Kodya Semarang. Penilaian DA dengan Hanifin & Rajka, derajad lesi dengan indeks ADSI dan koloni dengan kultur pada media agar darah. Sampel diambil dari lesi DA selnas diameter 1cm2 dengan menggunakan kapas steril kemudian dilakukan kultur pada media agar darah untuk mendapatkan jumlah koloni. Satu koloni dari S aureus diambil untuk pemeriksaan toksin SE-B dengan metode imunodifusi (Ouchterlony). Basil penelitian pada 14 penderita DA didapatkan hasil bahwa tidak ada hubungan antara derajad lesi DA dengan jumlah koloni S.aureus. Tidak ada hubungan antara derajad lesi DA dengan titer toksin SE-B.
Item Type: | Thesis (Masters) |
---|---|
Subjects: | R Medicine > R Medicine (General) |
Divisions: | School of Postgraduate (mixed) > Master Program in Biomedical Science |
ID Code: | 14882 |
Deposited By: | Mr UPT Perpus 2 |
Deposited On: | 18 Jun 2010 13:43 |
Last Modified: | 18 Jun 2010 13:43 |
Repository Staff Only: item control page