EFEK PEMBERIAN LIGNIN, CELLULOSA & AMORPHOPHALLUS ONCOPHYLLUS TERHADAP GAMBARAN HISTOPATOLOGIK KOLON TIKUS WISTAR (Penelitian eksperimental laboratorik pada tikus Wistar yang diinduksi 1,2 DMH subkutan, diet tinggi lemak dan protein)

Abstract

Background: In America, colon cancer is the 3rd rank cause of death, and the incidency in Indonesia is the 5th rank. 1,2 Dimethylhidrazine (1,2 DMH) is a potent carsinogens induce colon carsinogensesis. Iles-iles or A. oncophyllus contains lignin as a potent antioxidant that stimulate cell apoptosis, glukomannan as natural fiber with high viscosity and fiber itself. This research was directed to prove the anticarsinogenic-effect of lignin, cellulosa and A. oncophyllus in colon carcinogenesis by examining the differences of histopatological patterns. Methods: This research used randomized post test control group, using 36 male Wistar as sample, divided into 4 groups. Group I, 9 Wistar was control. Group II, 9 Wistar given lignin. Group III, 9 Wistar given cellulosa. Group IV, 9 Wistar given A. oncophyllus; group II,III,IV given carsinogens (1,2 DMH subcutan 1 mg/Wistar/week). A. onchophyllus dietary was given during carsinogenic induction for 8 weeks. At the beginning of the 9th weeks, the histopathological sample was made and scored by a pathologist based on the histopathological differences among colon epithelial mucous from WHO standard of classification. Data were analysed using Kruskall Wallis test. Results: Mean in group I was 1.53; group II was 1.13; group III was 1.22; and group IV was 1. Score 1=hyperplasia, score 2=mild dysplasia. The standard deviation (SD) of group I was ±0.518; group II was ±0.354; and group IV was ±1 . The Kruskall-Wallis test between groups was p=0,011, or significally different. The Mann-Whitney test between group I vs II p=0,046, I vs III p=0,000, and I vs IV p=0,009 or significally different. But the Mann-Whitney test between group II vs IV was p=0,317 or not significally different. Conclusion: There were no any differences in colon histopathological pattern between Wistar given lignin continued with carcinogens; Wistar given cellulosa continued with carcinogens and Wistar given A. onchophyllus followed with carcinogens. So, it may be concluded that lignin, cellulosa and A. onchophyllus blocked the colon carcinogenesis equally.