PENGARUH PEMBERIAN KATEKIN TEH HIJAU TERHADAP JUMLAH LEKOSIT DAN NETROFIL

Abstract

Background: Catechin is an active component of green tea that is potensial to strengthen body immunity system by increasing the secretion of Interleukin-12, phagositosis action, and the endurance and proliferation of lymphocyte. Other catechins extracted from SSD could increase Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) and Interleukin-6 (IL-6). GM-CSF and IL-6 could stimulate the proliferation of Hematopoetic Stem Cell (HSC), therefore neutrophil, eosinophil, basophil, and monocyte count would increase. Thus, there had not any researches been done about the effect of green tea catechins on the hematopoetic system. This study is aimed to know the effect of green tea catechins on leukocyte and neutrophil count in healthy human. Methods: This study was a Quasi Pre and Post test design. To the number of 16 healthy person who fulfilled the inclusive criterias were taken their blood specimens and counted leukocyte and neutrophil count before and after 4 weeks treatment with 350 mg green tea catechins capsule twice a day. From the peripheral blood smear were counted leukocyte count by Neubauer Improved counting chamber. Neutrophil count were counted by differential counting method from peripheral blood smear. The data were processed by SPSS 13.00 for Windows, using Wilcoxon test and Paired T-test. Results: Shapiro-Wilk test showed that leukocyte count data were not normally distributed (p<0,05). The Wilcoxon test were not significantly difference in leukocyte count before and after treatment, p=0,134 (p>0,05). The Shapiro-Wilk test for netrophil count were normally distributed (p≥0,05). Paired Sample T-Test showed that not significantly difference in neutrophil count before and after treatment, p=0,853 (p>0,05). Conclusion: Green tea treatment could not increase leukocyte and neutrophil count in healthy human.