ANTIBACTERIAL ACTIVITY OF A SECONDARY METABOLITE-PRODUCING CORAL BACTERIUM Pseudoalteromonas SPECIES

Karna Radjasa, Okky and Marten, Torben and Brinkoff, Thorsten and Grossart, Hans-Peter and Sabdono, Agus and Simon, Meinhard (2004) ANTIBACTERIAL ACTIVITY OF A SECONDARY METABOLITE-PRODUCING CORAL BACTERIUM Pseudoalteromonas SPECIES. Journal of Coastal Development , VII (2). pp. 78-88. ISSN ISSN: 1410-5217

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Abstract

A bacterium, collected at the surface of coral Acropora sp., TAB4.2 was successfully screened for secondary metabolites production based on PCR amplification of the non-ribosomal peptide synthetase gene. It was identified as closely related to Pseudoalteromonas luteoviolacea based on its 16S rDNA. TAB4.2 was found to inhibit the growth of all 5 coral-associated and all 5 pathogenic bacteria tested. To characterize the inhibiting metabolite, a 279 bp long DNA fragment was obtained and the deduced amino acid sequence showed conserved signature regions for peptide synthetases and revealed a high similarity to NosD (40 % identity), a multifunctional peptide synthetase from Nostoc sp. GSV224, and NdaB (44 % identity), a peptide synthetase module of Nodularia spumigena.

Item Type:Article
Subjects:S Agriculture > SH Aquaculture. Fisheries. Angling
Q Science > Q Science (General)
T Technology > TC Hydraulic engineering. Ocean engineering
Q Science > QK Botany
Divisions:Faculty of Fisheries and Marine Sciences > Department of Marine Science
ID Code:146
Deposited By:Mr. Wahyu Setyadi
Deposited On:06 May 2009 16:15
Last Modified:06 May 2009 16:15

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