Peran Limfosit T Helper-1 (TH1) dan T Helper-2 (TH2) pada Patogenesis Artritis Lepra

Abstract

The role of lymphocyte T Helper-1 (TH1) and T Helper-2 (TH2) in the pathogenesis of leprosy arthritis Background: The autoreactive of TH CD4+ cells is the thought to play an important role in arthritis leprosy pathogenesis. However, wheter of TH1 or TH2 predominant has never been studied. Methods: Various Ag M. leprae (Ag 35 kDa, 10 kDa, 45 kDa, 85 kDa, and MLSA 2 ug/ml) were stimulated to the peripheral blood (10cc) lymphocyte culture (PBMC) using 96 weels microplate and RPMI 1640 media of 22 leprosy arthritis cases, control-1 (n=12) (leprosy without arthritis) and control 2 (n=12) (healthy contact). The activity difference between TH1 and TH2 CD4+ lymphocyte was analysed using the difference delta levels of IFN-􀈖 and IL-4 (ELISA) of the three group studies. Statistical analysis used were ANOVA, Kruskal Wallis or Mann Whitney, and Chi-square. Results: IFN-􀈖 delta levels was significantly higher in the lymphocytes cultures in LA group (the median 132.234 pg/ml, 60.347 pg/ml, 14.093 pg/ml, 16.619 pg/ml and 138.394 pg/ml) compared with IL-4 value level (median 0.317 pg/ml, 0.017 pg/ml, -0.206 pg/ml, -0.200 pg/ml and 0.492 pg/ml) after being stimulated with 35 kDa, 10 kDa MMP-1, 45 kDa LAM, 85 kDa and MLSA of 2 ug/ml dose consecutively (all p<0.001). The IFN-􀈖 delta value in LA group also showed the significantly higher level in response to all M. leprae Ag compared to all control-groups, with all p value < 0.05. Conclusion: TH1 CD4+ lymphocyte activity is more dominant compared than TH2 CD4+ lymphocyte activity in leprosy arthritis group patients