SCREENING OF SECONDARY METABOLITE-PRODUCING BACTERIA ASSOCIATED WITH CORALS USING 16S rDNA-BASED APPROACH

Karna Radjasa, Ocky and Sabdono, Agus (2003) SCREENING OF SECONDARY METABOLITE-PRODUCING BACTERIA ASSOCIATED WITH CORALS USING 16S rDNA-BASED APPROACH. Journal of Coastal Development , 7 (1). pp. 11-19. ISSN ISSN: 1410-5217

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Abstract

A total of 42 coral-associated bacteria were screened for their ability to produce secondary metabolites by using 16S rDNA-based approach. A specific oligonucleotides primer was used to amplify DNA non-ribosomal peptide synthetase (NRPS). Five strains were found to carry the NRPS gene and successfully inhibit the growth of other coral-associated and pathogenic bacteria. The phylogenetic characterization revealed that four strains belonged to currently known species Pseudoalteromonas luteoviolacea, Vibrio tubiashi, Vibrio carchiariae and Bacillus iodinum. On the other hand, one strain was apparently closely related with an unknown group of the gamma Proteobacteria, which was only recognized as uncultured clone 141H2.

Item Type:Article
Subjects:T Technology > TC Hydraulic engineering. Ocean engineering
Q Science > Q Science (General)
S Agriculture > SH Aquaculture. Fisheries. Angling
Q Science > QK Botany
Divisions:Faculty of Fisheries and Marine Sciences > Department of Marine Science
ID Code:136
Deposited By:Mr. Wahyu Setyadi
Deposited On:04 May 2009 16:57
Last Modified:04 May 2009 16:57

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