PENGARUH SINAR ULTRAVIOLET B, DAN HORMON GENOTROPIN® PADA BIAKAN FIBROBLAS JARINGAN KULIT MANUSIA, KAHAN DENGAN A GYROPHILICNUCLEOLAR ORGANIZER REGION DAN FIBROBLAST POPULATED COLLAGEN LATTICE

Abstract

Solar UV rays exposure plays a pivotal role in skin aging. It causes extracellular collagen matrixs damage and defective collagen synthesis in cellular level. One way to fight aging is by giving hormone replacement therapy using Human Growth Hormone. Several recombinant products have been developed, one of these is genotropin® hormone. In several in vivo studies, genotropin® has been shown to delay aging, but in vitro studies to investigate the ability of genotropin® to fight aging process in fibroblast culture have never been performed. This study seeks to assess the role of genotropin® in inhibiting aging process caused by UVB exposure towards fibroblast proliferation and collagen synthesis in fibroblast culture taken from normal human skin tissue. The assessment was performed by measuring the value of proliferation index in Agyrophylic Nucleolar Organizer Region (AgNOR) and contraction levels on Fibroblast Populated Collagen Latice (FPCL). This was a laboratory experimental study using posttest-only control group design with cells cultured in cell culture wells as the research unit samples. The samples were exposed to UVB with doses of 10, 20 and 30 mJ/cm2 and genotropin® with doses of 30, 50 and 100 plU/mL in fibroblasts cultured within collagen network. The fibroblasts were derived from cultured healthy human skin tissue taken from the back of an 18 years old donor. Collagen synthesis underwent significant reduction when exposed to UVB radiation with increasing doses. On the other hand, the sample groups given genotropin® with increasing doses showed increased collagen synthesis. This also occurred to combination treatment groups, which were the groups receiving genotropin® with increasing doses and UVB radiation dose of 20 mJ/cm2 and 30 mJ/cm2 if compared with control groups. Besides, increasing doses of genotropin® hormone influenced the fibroblast proliferation index compared to the control group. The greater the dose of genotropin® hormone, the higher the fibroblast proliferation index compared to control groups. Sinar ultraviolet matahari berperan dalam menyebabkan penuaan kulit, yaitu mengakibatkan kerusakan kolagen matriks ekstraseluler dan kerusakan sintesis kolagen pada tingkat seluler. Terapi sulih hormon dengan genotropin® adalah salah satu cara untuk menunda penuaan. Terdapat beberapa penelitian in vivo genotropin terbukti dapat menunda penuaan, akan tetapi penelitian in vitro untuk melihat kemampuan genotropin® menunda penuaan terhadap biakan kultur fibroblas belum pemah dilakukan. Penelitian ini ingin mengetahui peranan genotropin® dalam menunda proses penuaan yang disebabkan paparan UVB terhadap proliferasi fibroblas dan sintesis kolagen pada biakan kultur fibroblas dari jaringan kulit manusia. Penilaian dilakukan dengan menghitung nilai indek proliferasi pada Agyrophylic Nucleolar Organizer Region (AgNOR), dan deraj at kontraksi pada Fibroblast Populated Collagen Latice (FPCL). Dengan rancangan penelitian eksperimental laboratorik, pendekatan posttest-only control group design menggunakan kultur sel dalam sumuran sebagai sampel unit penelitian. Sel fibroblas berasal dari kultur jaringan kulit manusia dari seorang donor. Diberikan perlakuan paparan sinar ultraviolet- B dosis 10 mJ/cm2, 20 mJ/cm2, 30 mJ/cm2 dan genotropin® dosis 30 plUhnl, 50 plU/m1 dan 100 pIII/m1 pada biakan kultur fibroblas di dalam kisi-kisi kolagen.. Hasilnya sintesis kolagen mengalami penurunan bermakna ketika mendapat paparan UVB dengan dosis semakin meningkat. Sebaliknya kelompok paparan genotropin® dengan dosis yang semakin meningkat, sintesis kolagen juga semakin meningkat. Demikian juga terhadap kelompok yang mendapat perlakuaan kombinasi dosis genotropin® yang semakin meningkat dan UVB dibandingkan dengan kelompok kontrol. Selain itu ada pengaruh paparan hormon genotropin® dosis semakin besar terhadap indeks proliferasi fibroblas bila dibandingkan dengan kelompok kontrol. Setnakin besar dosis paparan hormon genotropin®, indeks proliferasi fibroblas semakin meningkat bila dibandingkan kelompok kontrol.