ALTERATION OF RAT REPRODUCTIVE ORGAN IN ADULTHOOD CAUSED BY THE EXPOSURE OF FOREIGN ESTROGENIC COMPOUNDS (MOSQUITO INSECTICIDES) DURING EARLY LIFE

Abstract

Background : Over the past decades, there has been increasing concern about the impact of environmental estrogen that act like hormone in human reproductive development and health. Its compounds may occur not only through industrial and agricultural activities, but also come from household product such as insecticides. Objectives : to elucidate the clinical effect of proposed estrogenic compound during young period of male rat on inducing alteration of reproductive organ development in adult Methods : Design of this study is true experimental design with the post test only control group design. Newborn male SD rats (n=88) were randomised allocated into 6 groups (negative control (n=15), positive control (n=11), estrogen 25 1.1g (n=15), burn insecticides (n=18), liquid insecticides 3 ml (n=20), and liquid insecticides 4 ml (n=9)). Treatment was given for 20 days. At 100th days old, all rats were killed, testis, penis and prostate gland were removed for further measurement. Recorded data were testis volume and weight, penis length and diameter. Histopathological section of prostate gland were examined under HE and Masson's Trichrome staining. Result : 13-estradio1-3-benzoat (a high potent estrogen) treatment in alternate days for 20 days caused significant lower testicle volume (0.3±0.08 ml) and weight (0.66±0.17 mg), shorter (0.84±0.08 cm) and smaller (0.29±0.05 cm) penis compare to positive control group (p<0.05). Exposure of burn mosquito insecticides caused significantly exhibit shorter (1.1±0.07 cm) and smaller (0.4±0.03 cm) of penis compare to negative control group (p<0.05). Exposure of 4 ml liquid insecticides caused significant smaller (0.41±0.03) penis diameter compare to negative control group (p<0.05). Both 13 estradiol 3-benzoat and foreign estrogenic compunds caused alteration of histopathological characteristics of the prostatic gland in adulthood. Conclusion : In general, exposure of burning mosquito insecticide coil, liquid mosquito insecticide and administering of 25 12g p estradiol 3-benzoate to neonatal male SD rat for 20 days can cause alteration of reproductive organ in adult.