PERBANDINGAN SEKRESI IL — 10 DI JARINGAN SEKITAR LUKA DENGAN DAN TANPA INFILTRASI LEVOBUPWAKAIN'PADA NYERI PASCA INSISI Studi Imunohistokimia pada Tikus Wistar COMPARISON OF IL-10 SECRETION OF LEVOBUPIVACAIN AND NON LEVOBUPIVACAIN TREATED TISSUE SURROUND WOUND AFTER INCISION PAIN Immunohistochemistry Study on Wistar rats

Winarto, Winarto (2005) PERBANDINGAN SEKRESI IL — 10 DI JARINGAN SEKITAR LUKA DENGAN DAN TANPA INFILTRASI LEVOBUPWAKAIN'PADA NYERI PASCA INSISI Studi Imunohistokimia pada Tikus Wistar COMPARISON OF IL-10 SECRETION OF LEVOBUPIVACAIN AND NON LEVOBUPIVACAIN TREATED TISSUE SURROUND WOUND AFTER INCISION PAIN Immunohistochemistry Study on Wistar rats. Masters thesis, Program Pasca Sarjana Universitas Diponegoro.

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Abstract

Backaround : The acute pain occurs after wound could depress the immune function that leads to the inhibition of the wound healing processes. Local anestetic which has a long duration effect such as levobupivacain could be used to relief the pain so that protect the depression of immune function. Activation of macrophage can promote the T cell to produce IL-10. Ob'ective : To compare the 11,-10 secretion between levobupivakain tread and non levobupivacain on the tissue surround the wound. Methode : This laboratoric experimental study was designed with randomized post test only control group method. Thirty five female rats were randomly devided in to three groups. Control group (K), Non-Levobupivacain infiltration group (P1) and Levobupivakain infiltration group (P2). No incision and no infiltration in K group. Two cm of skin incision was perfomed to P1 and P2. After the incision, Levobupivacain infiltration were given every 8 hours for 24 hours to the P2 group. No levobupivacain was given to the first group. On day 1st,2nd:th the rats were sacrified and the tissue surround the wound were taken for immunohistochemistry stainning. The IL-10 secretion were analyzed for histologic scoring. Kruskal Wallis Test, Marm-Whitney Test were used for statistic analysis Result : It was demonstrated in this study that the histologic score of IL-10 of the levobupivacain treated group (group 2) was signicicantly higher than group 1 (without levobupivacain) in 2fid day (mean 5.36±1.25 vs 3 00 ±2 11 respectively, p=0,023 ; p<0,05). Conclusion : The IL-10 secretion was significanly higher in levobupivacain treated group than non levobupivacain group on the tissue surround the wound Latar belakang : Nyeri menyebabkan peningkatan hormon glukokortikoid yang memperlama penyembuhan luka. Transmisi nyeri dapat dihambat dengan obat anestesi lokal levobupivakain. Terapi ini akan mengurangi supresi imunitas seluler sehingga fungsi makrofag dalam membantu aktifasi sel T tidak terhambat. Aktifasi sel T ini diduga akan meningkatkan sekresi IL-10. Tu'uan Membandingkan sekresi IL-10 di jaringan sekitar luka dengan dan tanpa infiltrasi levobupivakain. Metode : Dilakukan penelitian eksperimental laboratorik dengan disain "Randomized Post test only control group design", pada tigapuluh lima ekor tikus Wistar. Kelompok penelitian dibagi menjadi tiga kelompok secara acak, Kelompok Kontrol (K) lima ekor, Perlakuan 1 (P1) dan Perlakuan 2 (P2) masing-masing limabelas ekor. Kelompok Kontrol, tikus tanpa insisi dan tanpa infiltrasi. Kelompok P1, tikus yang dilakukan insisi 2 cm, tanpa diberikan infiltrasi levobupivakain, Kelompok P2, tikus yang dilakukan insisi 2 cm, diberikan infiltrasi levobupivakain tiap 8 jam selama 24 jam. Ekspresi IL-10 di sekitar luka insisi dinilai dengan skor histologi dari preparat dengan menggunakan pengecatan secara imunohistokimia, yang diambil dari biopsi jaringan pada hari ke 1,2 dan 3. Metode perhitungan statistik menggunakan Kntslcal Wallis Test dilajutkan Mann-Whitney Test. Hasa : Dari hasil penelitian menunjukkan pada jaringan insisi rerata skor histologi IL-10 pada kelompok levobupivakain lebih tinggi (5.36±1.25) dibanding kelompok tanpa levobupivakain (3 00 ±2 11) pada hari ke dua. Perhitungan statistik antara kedua kelompok tanpa levobupivakain dan dengan kelompok levobupivakain berbeda bermakna (p-1.023 ; p<0.05). Kesimpulan Sekresi IL-10 di jaringan sekitar luka dengan infiltrasi levobupivalcain lebih tinggi dibanding tanpa levobupivakain pada hari kedua.

Item Type:Thesis (Masters)
Subjects:R Medicine > R Medicine (General)
Divisions:Postgraduate Program > Master Program in Biomedical Science
ID Code:12537
Deposited By:Mr UPT Perpus 1
Deposited On:31 May 2010 15:49
Last Modified:31 May 2010 15:49

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