Sadad, Arif Rahman (2004) PERBEDAAN HASIL PREPARASI FRAGMEN DNA MITOKONDRIAL DAN DNA INTI PADA SAMPEL URIN SIMPAN UNTUK ICEPENTINGAN IDENTIFIKASI PERSONAL. Masters thesis, Program Pendidikan Pasca sarjana Universitas Diponegoro.
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Abstract
Backgrounds: The identification of a person in various forensic cases may use both conventional and modern methods (DNA typing). DNA investigation has an advantage because DNA is more stable as compared with conventional genetic marker methods (for example, blood group), and DNA genetic codes are always differ from person to person, except for identical twin. Recently, personal identification by using urinary sample is beginning to play a sufficiently important role. In examinations of doping cases and narcotics abuse that are performed using urinary sample that gives positive results, the user often denies the result. Other than nuclear DNA, in 1981 the mitochondrial DNA (mtDNA) was discovered, i.e. DNA that resides in mitochondrion. Every cell has 1000 to 10,000 copies of mtDNA, so the mtDNA can be found in an extremely small amount of sample, and urinary sample is included among them. Methods: An analytic cross-sectional study was performed that involved 12 selected respondents (7 females and 5 males) from all age groups, urinary samples were collected for performing DNA fragmentation (mtDNA and nDNA) and blood samples were collected as reference sample (both mtDNA and nDNA). Description of difference of mtDNA and nDNA fragment preparation results was performed (both blood and urine), then the difference of fragment preparation result between mtDNA and nDNA in the urinary samples was analysed. Results: The result of blood mtDNA and nDNA fragment preparation was found to be 100%, whereas urinary mtDNA was 83,3% and urinary nDNA was 33,3%. From the analysis of difference between urinary mtDNA fragment preparation result as compared with urinary nDNA was found to be 83.3%:33.3% with p = 0.031. So the result of urinary mtDNA fragment preparation was proved to be 2.5 times higher than urinary nDNA fragment preparation. Conclusion: Because the result of urinary mtDNA fragment preparation is higher than urinary nDNA fragment preparation, so in cases of narcotic abuse or doping with urine as the sample, then the mtDNA investigation should be used to find out the owner of the urine. Latar belakang : Identifikasi seseorang pada berbagai kasus forensik dapat menggunakan cara konvensional maupun modern ( DNA typing ). Pemeriksaan DNA mempunyai keunggulan karena DNA bersifat lebih stabil dibanding metode petanda genetik konvensional ( misalnya golongan darah ), dan kode genetik DNA pada setiap individu pasti berbeda, kecuali pada kembar identik. Identifikasi personal dengan menggunakan sampel urin akhir-alchir ini mulai memegang peran cukup penting. Pada kasus doping dan pemeriksaan narkoba yang menggunakan sampel urin dengan basil yang positif seringkali terjadi pengingkaran oleh pengguna. Selain DNA inti, pada tahun 1981 ditemukan DNA mitokondrial ( mtDNA ), yaitu DNA yang terdapat pada mitokondria. Setiap sel mempunyai 1000 hingga 10.000 copy mtDNA, sehingga mtDNA dapat ditemukan pada sampel yang sangat sedikit termasuk diantaranya adalah sampel urin. Metoda : Dilakukan 'studi cross — sectional analitik dengan melibatkan 12 responden terpilih ( 7 perempuan dan 5 laki-laki ) dari semua golongan umur yang diambil sampel urin untuk dilakukan preparasi fragmen DNA ( mtDNA dan nDNA ) dan dilakukan pengambilan darah sebagai sampel referensi ( baik mtDNA maupun nDNA ). Dilakukan diskripsi perbedaan basil preparasi fragmen mtDNA dan nDNA ( balk darah maupun urin ), kemudian dianalisis perbedaan hasil preparasi fragmen mtDNA dibanding nDNA pada sampel urin. Basil : Didapatkan basil preparasi fragmen mtDNA dan nDNA darah sebesar 100%, mtDNA urin sebesar 83,3% dan nDNA urin sebesar 33,3%. Dan analisis perbedaan basil preparasi fragmen mtDNA dibanding nDNA win didapatkan 83,3%:33,3% dengan p=0,031. Sehingga basil preparasi fragmen mtDNA urin terbukti 2,5 kali lebih tinggi dari preparasi fragmen nDNA urin. Simpulan : Karena hasil preparasi fragmen mtDNA urin lebih tinggi dari preparasi fragmen nDNA urin maka pada kasus-kasus narkoba atau doping dengan urin sebagai sampelnya sebaiknya digunakan pemeriksaan mtDNA untuk mengetahui kepemilikan dari urin tersebut.
Item Type: | Thesis (Masters) |
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Subjects: | R Medicine > R Medicine (General) |
Divisions: | School of Postgraduate (mixed) > Master Program in Biomedical Science |
ID Code: | 12347 |
Deposited By: | Mr UPT Perpus 1 |
Deposited On: | 30 May 2010 14:51 |
Last Modified: | 30 May 2010 14:51 |
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