PENGARUH BERBAGAI JENIS KUNING TELUR SEBAGAI BAHAN BAKU MEDIUM TERHADAP MOTILITAS, VITALITAS DAN MORFOLOGI SPERMATOZOA MANUSIA SELAMA PROSES SIMPAN BEKU Effect of various egg yolks in cryopreservation medium on motility, vitality and morphology of cryopreserved human spermatozoa

Abstract

The present study were conducted to evaluate effect of various egg yolks in cryopreservation medium on motility, vitality and morphology of human spermatozoa by comparing differences motility, vitality, and morphology of spermatozoa that cryopreserved in modified TES-Tris yolk citrat (m-TESTYC) medium with hen's egg yolk, duck's egg yolk, goose' egg yolk, and modified TES-Tris yolk citrat (m-TESTYC) without egg yolk. Semen were collected by masturbation after 3 - 5 days abstinences from 30 adult males. The parameters taken into consideration were motility, vitality, and morphology of spermatozoa. All of the sample were divided into four groups. First group was added with mTESTYC medium without egg yolk (as control group/A group), second group was added with m-TESTYC which contain hen's egg yolk (as B group), third group was added with m-TESTYC which contain duck's egg yolk (as C group), fourth group was added with m-TESTYC which contain goose's egg yolk (as D group). Ratio of medium and semen samples were 1 : 1. The samples were plunged into liquid nitrogen at - 196 °C and stored for two weeks ant then removed from the liquid nitrogen and thawed at room temperature. Motility, vitality and morphology of the spermatozoa after thawed were examined immediatly. The study was adapts to randomized pre-test and post test control group design. Data were analyzed with Kruskal Wallis and Mann-Whitney U test. The results showed motility and vitality of the spermatozoa that cryopreseved in m-TESTYC medium with hen's egg yolk (B group) was the highest, but there were not significantly difference on morphology of the post freezing spermatozoa among treatment groups. Penelitian ini dilakukan untuk mengevaluasi pengaruh dari berbagai kuning telur dalam medium simpan beku pada motilitas, vitalitas dan morfologi spermatozoa manusia dengan membandingkan perbedaan motilitas, vitalitas, dan morfologi spermatozoa yang disimpan beku dengan menggunakan medium modified TES-Tris yolk citrat (m-TESTYC) dengan kuning telur ayam, kuning telur bebek, kuning telur angsa dan medium modified TES-Tris yolk citrat (m-TESTYC) tanpa kuning telur. Sampel yang digunakan pada penelitian ini berasal dari 30 pria dewasa yang dikoleksi dengan cara masturbasi setelah abstinensi selama 3 - 5 hari. Parameter yang diamati meliputi motilitas, vitalitas dan morfologi spermatozoa. Seluruh sampel kemudian dibagi menjadi 4 kelompok dan ditambahkan medium m-TESTYC tanpa kuning telur (blanko) sebagai kontrol (perlakuan A), medium m-TESTYC dengan kuning telur ayam (perlakuan B), medium m-TESTYC dengan kuning telur bebek (perlakuan C), medium m-TESTYC dengan kuning telur angsa (perlakuan D). Penambahan medium dengan perbandingan 1 : 1. Semua sampel disimpan dalam nitrogen cair pada suhu - 196 °C selama 2 minggu, kemudian dilakukan thawing pada temperatur kamar. Setelah dilakukan thawing motilitas, vitalitas dan morfologi spermatozoa segera diamati. Rancangan penelitian ini menggunakan Randomized pre-test and post-test control group design. Data yang terkumpul diuji dengan uji Kruskal Wallis dan dilanjutkan dengan uji Mann-Whitney U Pada penelitian ini hasil tuaian spermatozoa yang disimpan beku dalam medium m-TESTYC dengan kuning telur ayam dapat mempertahankan motilitas dan vitalitas tertinggi dari seluruh kelompok perlakuan, sedang morfologi spermatozoa post freezing ternyata tidal berbeda bermakna antar kelompok perlakuan.