PENGARUH PEMBERIAN GONODERMA LUCIDIUM TERHADAP RESPON PRALIFERASI LIMFOSIT MENCIT YANG DIANOKULASI SALMODELLA TYPHIMURIUM

Abstract

Ganoderma lucidum had been proved to act as immunomodulator. It could also increase IL-2 secretion and stimulates CD4+ T expression. The objective of this study was has to improve the effect of Ganoderma lucidum on Lymphocyte proliferation response of mice which were inoculated Salmonella typhimurium. This study was design as " The Posttest Only Control Group Design". Twenty four BALB/c male mice were divided into 4 groups randomly. The first group was inoculated with S. typhimurium, the second group was given G. lucidum 4 mg/day orally, the third group was inoculated with S. typhimurium and given G. lucidum 1 mg/day orally and the fourth group was inoculated with S. typhimurium and given G. lucidum 4 mg/day orally. Ganoderma lucidum was given orally on the first day until day 18'h, the S. typhimurium was inoculated on day 15th. The Spleens, limphnodes and thymus were harvested on day 19th. The result of this study showed that Ganoderma lucidum could increase lymphocyte proliferation response. The lymphocyte and lymphoblast count of spleens, lymphnodes and thymus were increase significantly ( p < 0.001 ), but spleens weight was not increase significantly ( p > 0,05) . Key words : Ganoderma lucidum, lymphocyte proliferation, Salmonella typhimuriu Ganoderma lucidum merupakan jamur yang telah tezbukti sebagai imunomodulator, dimana dia dapat meningkatkan ekspresi sel T CD4+ dan meningkatkan produksi IL-2. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian Ganoderma lucidum terhadap respon proliferasi limfosit mencit yang diinokulasi Salmonella typhimurium. Digunakan rancangan penelitian "The Posttest Only Control Group Design". Dua puluh empat mencit jantan BALB/c dibagi secara acak menjadi 4 kelompok. Kelompok ke 1 mendapat S.typhimurium saja, kelompok ke 2 mendapat G.lucidum 4 mg/hari peroral , kelompok ke 3 mendapat G.lucidum 1 mg/hari peroral & S.typhimurium dan kelompok Ice 4 mendapat G.lucidum 4 mg/hari peroral & S.typhimurium. Pemberian G.lucidum dilakukan mulai hart pertama sampai dengan hari ke 18, pemberian S.typhimurium dilakukan pada hari ke 15. Pada hari ke 19 dilakukan pemeriksaan sampel yaitu berat limpa, jumlah limfosit limpa,limfonodi dan timus serta jumlah limfoblas per 200 sel limpa, limfonodi dan timus. Basil penelitian menunjukkan pada pemberian G.lucidum terjadi peningkatan respon proliferasi limfosit. Peningkatan jumlah limfosit dan jumlah limfoblas per 200 sel limpa, limfonodi dan timus sangat bermakna ( p < 0.001 ) tetapi peningkatan berat limpa mencit tidak bermakna ( > 0.05) .