PENGARUH PEMBERIAN EKSTRAK Phyllanthus niruri L PADA SEL MONONUKLEAR TERHADAP VIABILITAS SEL ADENOKARSINOMA MAMMA MENCIT C3H PENELITIAN INVITRO

Abstract

Breast cancer is the second rank on the incidence of cancer in Indonesia, whereas, cervical cancer is the first. Phyllanthus niruri Linn is one of traditional herbs which have immunostimulant effect Some studies showed that Phyllanthus niruri Linn has potential benefits to increase the lymphocyte-cell proliferation, NK cell cytotoxic activity and macrophage phagositosis. This aim of this resarch is to evaluate the influence of the added of different doses aquous extract of Phyllanthus niruri Linn into mononuclear cells to the viability of cancer cells. The design of this experimental laboratory research (invitro study ) is post test randomized control groups design. Cancer cells culture (adenocarcinoma mammae ) of C3H mice, were compared to mononuclear cells culture of healthy C3H mice, in a comparative range of 1: 50 of cancer cells and mononuclear cells. The mononuclear cells had been activated by employing the different doses aquaous extract of Phyllanthus niruri Linn ( 0,1 mg/ml, 1 mg/ml, 10 mg/ml, and 100 mg/ml ) for 24 hours, 48 hours and 72 hours. After that,the mononuclear cells in each goups of time were mixed with cancer cells for 18 hours. We compared the death and the living cancer cells to evaluate the viability of cancer cells. Analysis of Variance of Multifactor, Tukey HSD test and Thinnest test were applied to analyse the data. The risult suggest that the added of aquaous extrack of Phyllanthus niruri Linn into mononuclear cells decreased the viability of cancer cells . The result also showed that the viability of cancer cells is effectively decreased by employing the dose of 100 mg/ml Phyllanthus niruri Linn , in an activated time of mononuclear cells for 72 hours . In this group the viability of cancer cells was 32,1 %. Conclusion: Phyllanthus niruri Linn extract that added into mononuclear cells decrease the viability of cancer cells. Key word : Phyllanthus niruri L, cancer cells viability, adenocarsinoma mamma, C3H mice.